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. 2014 Aug 29;77(2):65–72. doi: 10.4046/trd.2014.77.2.65

Figure 4.

Figure 4

Effects of aqueous extract and 70% ethanolic extract of Morus alba L. on phorbol 12-myristate 13-acetate (PMA)-induced MUC5AC production from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of aqueous extract (AE) (A) and 70% ethanolic extract (EE) (B) of Morus alba L. for 30 minutes and then stimulated with PMA (10 ng/mL) for 24 hours. Cell lysates were collected for measurement of MUC5AC mucin production by enzyme-linked immunosorbent assay. Three independent experiments were performed and the representative data were shown. Each bar represents a mean±SEM of 3 culture wells in comparison with that of control set at 100%. Concentration unit is µM. *Significantly different from control (p<0.05). †Significantly different from PMA alone (p<0.05).