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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Stem Cells. 2014 Oct;32(10):2657–2667. doi: 10.1002/stem.1779

Figure 4. CNVs arising from predetermined loci.

Figure 4

(A) The same 275 kb deletion was identified as de novo events in F31 and F29-P12 (passage 12 of F29). The top of the panel illustrates aCGH plots delineating the deletions. In F29-P12, the 275 kb deletion is part of a larger complex rearrangement event, which includes a smaller deletion in the distal region. Below the array image are coordinates of the CNV region and an agarose gel picture of breakpoint PCR results showing the presence or absence of the breakpoint junction in the assayed samples. The results suggest that the mutations leading to the deletions in F31 and F29-P12 are both de novo events. Below the gel image is alignment of breakpoint sequence to reference sequences. Red and blue colors indicate alignment of the breakpoint sequence to the reference sequences, whereas purple indicates the interval of microhomology. (B) The same 1.4 Mb duplications were identified in both F3-P6 and F8 as independent de novo events. (C) The apparently same 699 kb duplications were identified in G5 and G30. (D) The same 337 kb deletions were identified in G23 and G10.