Table 1.
Number of neuronal, B cell, fibroblast iPSCs and neurospheres with CNVs
| Neuronal iPSC | B cell iPSC | Fibroblast iPSC | Neurosphere | |
|---|---|---|---|---|
| Embryo 1 | - | - | - | 2/26 |
| Embryo 2 | - | - | - | 0/27 |
| Embryo 3 | - | - | - | 1/23 |
| Embryo 4 | 1/12 | 4/13 | - | - |
| Embryo 5 | 0/5 | 1/5 | - | - |
| Embryo 6 | 0/3 | 5/13 | 0/1 | - |
| Embryo 7 | 0/3 | 1/7 | 1/9 | - |
| Total | 1/23 (4.3%) | 11/38 (29%) | 1/10 (10%) | 3/76 (3.9%) |
| Total excluding somatic mosaicism | 1/23 (4.3%) | 11/38 (29%)a | 1/10 (10%) | 1/76 (1.3%) |
a
the percentage may be overestimated here. This is because among the 14 CNVs identified by aCGH from the 11 B cell iPSC samples, breakpoint PCR was successful only for 9 CNVs. None of these 9 CNVs were detected to be present as a mosaic state in the corresponding fibroblast DNA by PCR. However, the possibility of the other 5 CNVs being somatic mosaicism cannot be ruled out.