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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Clin Virol. 2014 Jul 30;61(2):242–247. doi: 10.1016/j.jcv.2014.07.014

Figure 1. Detection of HEV in a single infected islet.

Figure 1

A human islet, infected for 72 h (A-C) or 12 h (D-F) was mixed with non-infected cultured pancreatic cells, a pancreatic tissue biopsy, or used alone for RNA extraction. RT-snPCR with primers in the 5’ untranslated region (B, E) or in the VP3-VP1 region (C, F) was used for detection of HEV genome. The products from the first (PCR#1) and seminested (PCR#2) reactions were analyzed on a 1.5% agarose gel containing SYBR® Safe. Immunohistochemical staining with an antibody against HEV VP1 was used for detection of HEV protein (A, D). A representative infected islet, infected for 72 h (A) or 12 h (D) is shown.