Figure 2. Degradation of HEV during incubation at 37°C.
A 105TCID50/200 μL stock of HEV was diluted 1:100 in fresh culture media, or cell-free pancreatic cell culture supernatant, and viral RNA was extracted directly, or after 24 h incubation at 37°C. The amount of viral RNA was semi-quantified with qPCR and the Cq values of three independent experiments are displayed on the vertical axis. Cq; quantification cycle.
