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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Protein Expr Purif. 2014 Aug 12;102:69–75. doi: 10.1016/j.pep.2014.08.005

Figure 1. pJ915-rhChymase vector map.

Figure 1

The P. pastoris expression plasmid pJ915-rhChymase (4035 bp) encodes a fusion protein that consists of rhChymase linked to yeast α-Mating Factor (a secretion signal peptide) by a kexin cleavage site. The cDNA for rhChymase with the kexin cleavage site was transferred from a previously reported plasmid, pPICzα-rhChymase [38] using 5’ XhoI and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.