Table 2.
Kinetic Parameters of (Man)5-rhChymase.
| KM (M) | kcat (sec−1) | kcat/KM (M−1 sec−1) | |
|---|---|---|---|
| Suc-AAPF-pNA | |||
| − Heparin | 9.35×10−4 ± 0.925×10−4 | 3.6 ± 0.1 | 3.85×103 ± 0.394×103 |
| + Heparin | 5.14×10−4 ± 0.533×10−4 | 2.5 ± 0.1 | 4.86×103 ± 0.394×103 |
| Suc-AAPF-SBzl | |||
| − Heparin | 3.55×10−5 ± 1.18×10−5 | 12.6 ± 0.9 | 3.55×105 ± 0.990×105 |
| + Heparin | 1.57×10−5 ± 0.612×10−5 | 17.8 ± 1.1 | 1.13×106 ± 0.388×106 |
Michaelis-Menten enzyme kinetics of (Man)5-rhChymase are reported for suc-AAPF-pNA and suc-AAPF-SBzl, with and without 0.01 mg/mL heparin. All reactions contained 0.1 M HEPES, 1 M NaCl, 10 % Glycerol, 0.02% NaN3, 10 % DMSO, pH = 7.5. Assays with suc-AAPF-SBzl also contained 1 mM DTNB. Values show mean ± S.D. of three independent replicates.