Figure 2. The majority of IFN-γ producing cells in K14E7 skin are CD8⁺ and CD4⁺ T cells.

Flow cytometric analysis of cells isolated from dermis and epidermis of K14E7 mice, stimulated with PMA and ionomycin. a,b Percentage of CD3⁺, CD3⁺CD8⁺ and CD3⁺CD4⁺ T cells, iNKT cells and γδ T cells of CD45⁺IFN-γ⁺ cells. Plots are representative of 4 independent experiments (2 mice per experiment), whose results are summarized in B. c Mean fluorescence intensity of IFN-γ-producing cell subsets from 4 independent experiments. Bars indicate means±SEM of 4 independent experiments.