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The effect of vitamin C on LPS-induced autophagy in macrophages. (a) MH-S cells were transfected with RFP-LC3 or GFP-LC3 plasmids for 24 hours. MH-S cells were cultured in the presence or absence of vitamin C and treated with LPS for 12 hours. (b) Western blotting of Beclin1; (c) western blotting of LC3. β-actin was probed as a loading control in (b) and (c).
