Figure 3. Effect of placebo and single dose of 20 g of ethanol on phosphorylation of MAPK isoforms in intestinal epithelium.

Mucosal protein was isolated from tissue lysates, and the levels of phosphorylated MAPK isoforms were determined by ELISA using anti-phosphospecific antibodies against p-P38 [A], p-ERK1/2 [B], and [C] and p-JNK. Lysates of Hela cells treated with Anisomycin were used as positive control (+ve control) for phosphorylated P38 and JNK, whereas A431 cells treated with recombinant human epidermal growth factor were used for phosphorylated ERK1/2. (n = 12 separate sample preparations; *P<0.05 vs. placebo).