(A): Te 85 clones were plated at a 105 cells/ml in 6 well plates in cell culture medium without FBS. Percentage of viable cells was evaluated at the different time points as described in materials and methods. The graph shows means ± SE, N = 12. *p<0.05, ***P<0.001 versus Te85 wt control; #
p<0.05 versus Te85 P2RX7A+B, $
p<0.05 versus Te85 P2RX7A. (B) Cell proliferation assessed for 24 hours in absence (untreated) or presence of: 4 U/ml Apyrase, 100 µM A740003, 100 µM BzATP, 100 µM BzATP +10 µM cyclosporin (CSA). A740003 and CSA vehicle (DMSO) was used as control. The graph shows means ± SE, N = 3. Colour coding: green Te85 wt, yellow Te85mock, red Te85 P2RX7A, cyan Te85 P2RX7B, purple Te85 P2RX7A+B.