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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Anesthesiology. 2014 Sep;121(3):510–527. doi: 10.1097/ALN.0000000000000278

Figure 1. Anesthesia with 3% sevoflurane two hours daily for three days, but not for one day, in P6 WT mice induces Tau phosphorylation in hippocampus of the mice.

Figure 1

A. Anesthesia with 3% sevoflurane two hours daily for three days in P6 mice increases the levels of AT8 (detected the levels of Tau-PS202 and Tau-PT205) in the hippocampus of the mice (harvested on P8) as compared to the control condition. There is no significant difference in β-Actin levels in the hippocampus of the mice between the sevoflurane anesthesia and control condition. B. Quantification of the Western blot shows that the sevoflurane anesthesia increases AT8(Tau-PS202 and Tau-PT205) levels in the hippocampus of the mice as compared to the control condition. C. The sevoflurane anesthesia does not increase the levels of total Tau in the hippocampus of the mice (harvested at P8) as compared to the control condition. There is no significant difference in β-Actin levels in hippocampus of the mice between the sevoflurane anesthesia and control condition. D. Quantification of the Western blot shows that the sevoflurane anesthesia does not increase the levels of total Tau in the hippocampus of the mice as compared to the control condition. E. Quantification of the Western blots (A and B) shows that the sevoflurane anesthesia increases the ratio of AT8(Tau-PS202 and Tau-PT205)/total Tau in the hippocampus of the mice as compared to the control condition. F. Anesthesia with 3% sevoflurane two hours daily for one day in P6 WT mice does not increase the levels of AT8(Tau-PS202 and Tau-PT205) in the hippocampus of the mice (harvested on P8) as compared to the control condition. There is no significant difference in β-Actin levels in the hippocampus of the mice between the sevoflurane anesthesia and control condition. G. Quantification of the Western blot shows that the sevoflurane anesthesia does not increase AT8(Tau-PS202 and Tau-PT205) levels in the hippocampus of the mice as compared to the control condition. H. Anesthesia with 3% sevoflurane two hours daily for one day does not increase total Tau levels in hippocampus of the mice (harvested on P8) as compared to control condition. There is no significant difference in β-Actin levels in hippocampus of the mice between the sevoflurane anesthesia and control condition. I. Quantification of the Western blot shows that the sevoflurane anesthesia does not increase the total Tau levels as compared to the control condition. J. Quantification of the Western blots (F and H) shows that the sevoflurane anesthesia does not increase the ratio of AT8(Tau-PS202 and Tau-PT205)/total Tau in the hippocampus of the mice as compared to the control condition. P, phosphorylated; Tau-PS202, Tau phosphorylated at serine 202; Tau-PT205, Tau phosphorylated at threonine 205. N = 6 in each group.