A. Anesthesia with 3% sevoflurane two hours daily for one day in P6 WT mice does not increase the levels of AT8(Tau-PS202 and Tau-PT205) and total Tau in the hippocampus of the mice (harvested on P6) as compared to the control condition. There is no significant difference in β-Actin levels in the hippocampus of the mice between the sevoflurane anesthesia and the control condition. B. Quantification of the Western blot shows that the sevoflurane anesthesia does not increase the ratio of AT8(Tau-PS202 and Tau-PT205) to total Tau in the hippocampus of the mice as compared to the control condition. C. Anesthesia with 3% sevoflurane two hours daily for three days in P6 mice increases the levels of AT8(Tau-PS202 and Tau-PT205), but not total Tau, in the hippocampus of the mice (harvested on P10) as compared to the control condition. There is no significant difference in β-Actin levels in the hippocampus of the mice between the sevoflurane anesthesia and the control condition. D. Quantification of the Western blot shows that the sevoflurane anesthesia increases the ratio of AT8(Tau-PS202 and Tau-PT205) to total Tau in the hippocampus of the mice as compared to the control condition. E. Anesthesia with 3% sevoflurane two hours daily for three days in P6 WT mice does not increase the levels of Tau-PS262 in the hippocampus of the mice (harvested on P8) as compared to the control condition. There is no significant difference in β-Actin levels in the hippocampus of the mice between the sevoflurane anesthesia and control condition. F. Quantification of the Western blot shows that the sevoflurane anesthesia does not increase Tau-PS262 levels in the hippocampus of the mice as compared to the control condition. P, phosphorylated; Tau-PS202, Tau phosphorylated at serine 202; Tau-PT205, Tau phosphorylated at threonine 205; Tau-PS262, Tau phosphorylated at serine 262. N = 6 in each group.