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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Exp Lung Res. 2014 Aug 25;40(8):415–425. doi: 10.3109/01902148.2014.938202

Figure 4. GFP expression in endothelial and leukocyte populations from bone marrow transplanted mice treated with bleomycin.

Figure 4

Mice were treated with intratracheal bleomycin and sacrificed on day 16. BrdU was administered 48 and 24 hours prior to harvest. Lungs were digested and single cell suspensions were evaluated for GFP expression to determine native tissue (GFP+) versus bone marrow (GFP-) origin. (A) Endothelial cells (ECs) were examined for BrdU positivity to identify the replicating endothelial cell population. This population was analyzed for GFP expression. (B) Leukocytes were identified as CD45 positive cells. This population was analyzed for GFP expression.