Fig. 6.

0.4 FiO₂ altered lung vascular patterning and VEGFR2 protein localization. Lungs that remained in RA for the entire culture period (a, b) developed apparent vascular tufts (green Lectin IF in (a) and robust VEGFR protein localization (arrows in b) that surrounded developing airways (*). However, lungs that were exposed to 0.4 FiO₂ for 48 h had decreased evidence of vessel formation (c) around airways (*) that had lost some of their structural integrity and had only isolated VEGFR2 positive cells (arrow in d). Epithelial boundaries are outlined by dashed lines in a and c to better identify airway structures in these immunofluorescent images. N ≥ 3 lungs/condition from at least 3 experiments