Fig. 8.

Hoxb5 or Hoxa5 over expression caused specific changes in VEGFR2 protein localization (a–c) and lectin-labeled vascular endothelial cells (green IF in d–f). Compared to control lungs (a, d), Hoxb5 over expression led to more prominent peri-airway VEGFR2 localization (b, arrow) and increased presence of endothelial cells (e, arrow) suggesting development of robust vessel clusters. Hoxa5 over expression (c) limited the degree of peri-airway VEGFR2 and the vascular pattern surrounding these airways was more simplified suggestive of limited vessel aborization (f, arrow). Mag. 40× (a–c), 20× (d–f), N = 2