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. 2014 Oct;95(Pt 10):2240–2250. doi: 10.1099/vir.0.066647-0

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Quantification of the packaging efficiency of mutant segments S4.14 and S4.15 in the reverse transcription packaging assay. Packaged S4 of BTV-9 was quantified by quantitative real-time PCR and correlated with the total amount of BTV cores using S6 as an internal control to determine the packaging efficiency. The efficiency for each mutant was then compared with the efficiency of the WT S4, considered as 100 %. Cells infected with BTV-1 but not transfected with S4 of BTV-9 (mock) were included as control. A difference between S4.14 and S4.15 was confirmed by Student’s t-test (P<0.001). Error bars: standard deviation from three independent experiments.