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Expression of VP4 from mutant S4 ssRNA. BSR cells were transfected with T7 transcripts of S4 of BTV-9 mutated in the 3′ UTR (S4.11, S4.12, S4.14, S4.15 and S4.16). As controls, WT S4 (B9) and mock-transfected cells were included. After 24 h, cells were fixed and stained with an anti-BTV-9 polyclonal serum. Bars, 200 µm.
