Fig. 7.

Prediction and confirmation of minimal sequence requirements for packaging of chimeric S4 segments. (a) Nucleotide sequence of chimeric segments. Boxed sequence represents S8 of BTV-9 ORF (grey box, S4.17 and S4.18) or flanking sequences from S4 of BTV-9 ORF (white box, S4.18). (b) RNAfold structure prediction of S4.17 and S4.18. (c) Reverse transcription PCR for S4 of BTV-9 (WT) without S4 (–ve), S4.17 or S4.18 from the CFA assay. Arrows indicate bands of 1981 and 1210 bp for mutant and WT ssRNAs, respectively. For consistency, each experiment was performed three times.