Figure 3.

M469I SNP detection in exon 10 of CFTR gene using RFLP-PCR reaction

A set of specific designed primers (CF-M469I-F and CF-M469I-R) were used for amplification of some part of exon 10 of the CFTR gene. The 240 bp amplified fragment was subjected to digestion with NdeI. The resultant fragments were separated on a 2.5% agarose gel. Different genotypes are identified according to the produced patter of the bands on the gel. A 50 bp DNA ladder was used as marker. I heterozygote infertile patient (GT: M469I), II uncut PCR product, III a normal homozygote GG person. Numbers are in base pair.