Figure 1. SAHA reduces glutamate secretion.

The amino acid secretome in gliomas cells was assessed by fully automatized ion-exchange chromatography analysis. Three well established glioma cell lines (human U87, rat F98, mouse GL261) were facilitated for this assay. The amino-acid serine was used as a reference and the glutamate/serine quotient calculated. SAHA silenced xCT-expression with an attendant, significant decrease in extracellular glutamate levels in all 3 cell lines already at 4 μM. Accordingly, there was no change in the extracellular concentration of the amino-acid cysteine. Statistical significance was calculated with the Student's t-test (mean ± sem, *P < 0.05, n = 3 per group).