Table 3.
Bacterial and fungal strains
| Stains | Characteristics | References |
|---|---|---|
| Burkholderia rhizoxinica HKI-0454 | Wild type, isolated from Rhizopus microsporus ATCC62417 | * |
| Rhizopus microsporus ATCC62417 | Fungal host harboring bacterial endosymbionts, isolated from rice seedlings | † |
| B. rhizoxinica ΔgspD::Kanr | T2SS mutant B. rhizoxinica with deletion of gspD | This study |
| B. rhizoxinica ΔgspC::Kanr | T2SS mutant B. rhizoxinica with deletion of gspC | This study |
| B. rhizoxinica Δchit::Kanr | B. rhizoxinica with deletion of chitinase gene | This study |
| B. rhizoxinica Δcbp::Kanr | B. rhizoxinica with deletion of chitin-binding protein gene | This study |
| B. rhizoxinica Δchts::Kanr | B. rhizoxinica with deletion of chitosanase gene | This study |
| B. rhizoxinica/pHKT4 | B. rhizoxinica wt harboring a RFP expression vector | This study |
| B. rhizoxinica ΔgspD::Kanr/pHKT2 | B. rhizoxinica gspD mutant harboring a GFP expression vector | This study |
| B. rhizoxinica ΔgspC::Kanr/pHKT2 | B. rhizoxinica gspC mutant harboring a GFP expression vector | This study |
| B. rhizoxinica Δchit::Kanr/pHKT2 | B. rhizoxinica chit mutant harboring a GFP expression vector | This study |
| B. rhizoxinica Δcbp::Kanr/pHKT2 | B. rhizoxinica cbp mutant harboring a GFP expression vector | This study |
| B. rhizoxinica Δchts::Kanr/pHKT2 | B. rhizoxinica chts mutant harboring a GFP expression vector | This study |
| R. microsporus + B. rhizoxinica ΔgspD::Kanr/pHKT2 | Reinfected cured fungal host with gspD mutant harboring a GFP expressing vector | This study |
| R. microsporus + B. rhizoxinica ΔgspC::Kanr/pHKT2 | Reinfected cured fungal host with gspC mutant harboring a GFP expressing vector | This study |
| R. microsporus + B. rhizoxinica Δchit::Kanr/pHKT2 | Reinfected cured fungal host with chit mutant harboring a GFP expressing vector | This study |
| R. microsporus + B. rhizoxinica Δcbp::Kanr/pHKT2 | Reinfected cured fungal host with cbp mutant harboring a GFP expressing vector | This study |
| R. microsporus + B. rhizoxinica Δchts::Kanr/pHKT2 | Reinfected cured fungal host with chts mutant harboring a GFP expressing vector | This study |
| Escherichia coli BL21(DE3)/pET28a-Chi | E. coli with expression vector harboring chitinase gene | This study |
*
Partida-Martinez and Hertweck (2005) Pathogenic fungus harbours endosymbiotic bacteria for toxin production. Nature 437:884–888.
†
Ibaragi (1973) Studies on rice seedling blight. I. Growth injury caused by Rhizopus sp. under high temperature. Ann. Phytopathol. Soc. Jpn 39:141–144.