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(A) Cysteine pairs within cross-linkable distances are indicated on the structure of Dyn1 (Protein Data Bank entry 3SNH). Mutants E341C/K694C and E482C/T676C also lay within the oligomerization interface, serving as positive controls for dimerization. (B) Nonreducing SDS–PAGE showing migration of cross-linked species obtained using MTS-1-MTS (3.6 Å) or MTS-11-MTS (∼16 Å).
