Figure 3.

Matrix-assisted laser desorption ionization (MALDI) Mega-time of flight analysis of cross-linked and non-cross-linked dynamin samples. (A) Cross-linking wild-type Dyn1 with MTS-2-MTS generates a minor high-molecular mass band (inset) that is readily detected as a dimer peak via MALDI mass spectrometry (201680 kDa) compared to non-cross-linked control (blue vs red). (B) Cross-linking Dyn^RCL(R15C/R730C) does not result in any change in the MALDI spectra (blue) compared to that of the un-cross-linked sample (red). The inset is a SDS–PAGE gel showing cross-linked and un-cross-linked proteins used for MALDI mass spectrometry.