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Average CD melting temperatures of TO-PNA-peptide:RNA duplexes from 25 to 95 °C were monitored at the peak wavelength 265 nm. (Violet) 12mer TO-PNA-peptide with fully complementary RNA, (Red) 12mer TO-PNA-peptide with single mismatch RNA, (Blue) 13mer TO-PNA-peptide with fully complementary RNA, (Magenta) 13mer TO-PNA-peptide with single mismatch RNA.
