Table 2. Melting Temperatures of TO-PNA-Peptide:RNA Duplexes^a.

complementary TO-PNA-peptide:RNA duplex		single mismatch TO-PNA-peptide:RNA duplex
duplex	Tm (°C)	duplex	Tm (°C)
TO1 (12mer): KRAS2 G12D	72.27 ± 0.25	TO1 (12mer): KRAS2 G12WT	64.30 ± 0.06
TO2 (13mer): KRAS2 G12D	70.14 ± 0.12	TO2 (13mer): KRAS2 G12WT	63.4 ± 0.47
TO3 (12mer): KRAS2 G12WT	73.23 ± 0.25	TO3 (12mer): KRAS2 G12D	60.10 ± 0.41
TO4 (13mer): KRAS2 G12WT	72.33 ± 0.12	TO4 (13mer): KRAS2 G12D	63.12 ± 0.36
TO5 (12mer): KRAS2 G12V	72.3 ± 0.25	TO5 (12mer): KRAS2 G12D	61.1 ± 0.56
TO6 (13mer): KRAS2 G12V	71.7 ± 0.21	TO6 (13mer): KRAS2 G12D	62.2 ± 0.36

^aCD thermal ramps from 25 to 95 °C were monitored at the peak wavelength, 265 nm. T_m of each duplex was determined from first derivative peaks ± standard deviation from n = 3 independent measurements.