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. 2014 Aug 11;25(9):1664–1677. doi: 10.1021/bc5002856

Figure 7.

Figure 7

Interaction of fluorescent molecules BODIPY FL vinblastine (4), BODIPY FL catharanthine (19), BODIPY FL vindoline (20), or sulfo-Cy5 vindoline (21) with 5 nM GST-hPXR-LBD and 5 nM Tb-anti-GST after 30 min of incubation. (A) Binding of indicated concentrations of BODIPY FL vinblastine (4) to either 5 nM Tb-anti-GST; 5 nM GST-hPXR-LBD in the presence of either DMSO (total binding) or TO901317 (1, 10 μM, background binding); or to 5 nM Tb-anti-GST, 5 nM GST, and DMSO (absolute background binding). (B) Binding of indicated concentrations of BODIPY FL catharanthine (19) to either 5 nM Tb-anti-GST; 5 nM GST-hPXR-LBD in the presence of either DMSO (total binding) or TO901317 (1, 10 μM, background binding); or to 5 nM Tb-anti-GST, 5 nM GST, and DMSO (absolute background binding). (C) Binding of indicated concentrations of BODIPY FL vindoline (20) to either 5 nM Tb-anti-GST; 5 nM GST-hPXR-LBD in the presence of either DMSO (total binding) or TO901317 (1, 10 μM, background binding); or to 5 nM Tb-anti-GST, 5 nM GST, and DMSO (absolute background binding). (D) Binding curves of indicated concentrations of sulfo-Cy5 vindoline (21) to either 5 nM Tb-anti-GST; 5 nM GST-hPXR-LBD in the presence of either DMSO (total binding) or TO901317 (1, 10 μM, background binding or nonspecific binding); or to 5 nM Tb-anti-GST, 5 nM GST, and DMSO (absolute background binding).