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. 2014 Aug 11;25(9):1664–1677. doi: 10.1021/bc5002856

Figure 8.

Figure 8

Binding activity of indicated concentrations of BODIPY FL vindoline (20) in the TR-FRET assay after 30 min of incubation with 5 nM Tb-anti-GST and other indicated assay components. (A) Interaction of BODIPY FL vindoline (20) with 5 nM GST-hPXR-LBD and 5 nM Tb-anti-GST in the presence of either DMSO or TO901317 (1, 10 μM); interaction of BODIPY FL vindoline (20) with 5 nM Tb-anti-GST and DMSO with or without 5 nM GST (without GST-hPXR-LBD). (B) Signal-to-background ratio of BODIPY FL vindoline (20) interaction with 5 nM GST-hPXR-LBD and 5 nM Tb-anti-GST, where signal and background are defined as 10 000 × 520 nm/490 nm ratios obtained from DMSO and TO901317 (1, 10 μM), respectively. For each BODIPY FL vindoline (20) concentration tested in both panels A and B, the difference between DMSO and TO901317 (1, 10 μM) was substantial and statistically significant (p < 0.0001).