Skip to main content
. 2014 Sep 17;8:299. doi: 10.3389/fnins.2014.00299

Figure 4.

Figure 4

Sex difference in neural projection from the LSi to MCG and effects of postnatal treatment with estradiol benzoate (EB) in the formation of the neural projection. Distribution of Fluoro-Gold (FG)-labeled neurons in the LS (A) and the number of FG-labeled neurons in the LSi (B) of female and male rats that received FG injection into the MCG. Female rats were subcutaneously injected with 50 or 100 μg of EB or vehicle on postnatal day 5 (day 1 = date of birth), and they were ovariectomized and treated with estradiol in adulthood (EB50, EB100, and control female groups). Male rats were castrated and treated with estradiol in adulthood. Each red dot of the line drawings of (A) indicates a FG-labeled neuronal cell body. Postnatal EB treatment dose-dependently decreased the number of FG-labeled neurons in the LSi of female rats, resulting in elimination of sex differences in the number of FG-labeled neurons. cc, corpus callosum; LV, lateral ventricle. *p < 0.05 vs. control female. (C) The mean LQ of control, EB50, EB100 female rats, and male rats. Postnatal EB treatment dose-dependently decreased lordotic activity of female rats (modified from Tsukahara et al., 2003).