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. 2014 Sep 17;34(38):12678–12689. doi: 10.1523/JNEUROSCI.1183-14.2014

Figure 2.

Figure 2.

gon-1 mutant protrusions form specifically at synapses, contain synaptic material, and are apposed to postsynaptic structures. A, Schematic of the DA9 motor neuron (in red). The DA9 cell body resides on the ventral side near the tail, and the DA9 axon projects to the dorsal side and then extends anteriorly, making synapses only in a specific region marked by the bracket. (The DA9 promoter used in B is also active in the adjacent VA12 neuron, shown in blue, which directly sends its axon anteriorly and makes synapses on the ventral side.) B, Pmig-13 driving expression of cytoplasmic mCherry and the synaptic active zone marker unc-10::GFP in gon-1(q518) mutants. Protrusions (arrows) are limited to the synaptic domain of DA9 (bracket) and contain accumulations of UNC-10::GFP. Scale bar, 20 μm. C, Pitr-1 driving expression of the synaptic vesicle marker rab-3::GFP in gon-1(q518) mutants. Protrusions contain accumulations of RAB-3::GFP. D, A loss-of-function mutation in the kinesin unc-104(e1265) does not suppress the protrusion phenotype in gon-1(q518) mutants (trIs30 marker). E, F, Labeling presynaptic DD motor neuron synapses with Punc-47::unc-10::TdTomato and postsynaptic muscles with Punc-129dm::nlg-1::YFP reveals that some postsynaptic material is also mislocalized away from the dorsal cord in gon-1(q518) mutants (F). Scale bar, 20 μm.