Figure 6.

Involvement of HDAC in MPP+-induced NF-κB suppression and cell death. A, MPP+ inhibits the transactivating activity of RelA. SH-SY5Y cells were cotransfected with the indicated plasmids in the presence of Gal4-tk-luciferase and pSV-β-galactosidase plasmids. After transfection, MPP+ (2 mm) and TSA (2 μm) were added for 12 h, and luciferase activity was normalized to β-galactosidase activity. B, Effect of TSA on MPP+-induced NF-κB activity. SH-SY5Y cells were cotransfected with plasmids NF-κB-luciferase and pSV-β-galactosidase, and treated with 2 μm TSA and 2 mm MPP+ for 12 h. C, SH-SY5Y cells were treated as indicated for 24 h, and cell survival was measured. *p < 0.05, **p < 0.01.