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. 2014 Sep 17;34(38):12725–12737. doi: 10.1523/JNEUROSCI.0985-14.2014

Figure 8.

Figure 8.

MiR-7 inhibits MPP+-induced NF-κB suppression by targeting RelA. A, Silencing RelA expression mitigates MPP+-induced NF-κB suppression. B, miR-7 lessens MPP+-induced NF-κB suppression. For A and B, SH-SY5Y cells were transfected as indicated above and treated with 2 mm MPP+ for 12 h. Plasmid pSV-β-galactosidase was also cotransfected for normalization of transfection efficiency. C, Silencing RelA expression inhibits MPP+-induced NF-κB suppression in differentiated ReNcell VM cells. These cells were transfected with NF-κB luciferase construct, infected with lenti-shRNA-RelA or lenti-shRNA-control vector, and treated with 1 mm MPP+ for 12 h. D, miR-7 expression inhibits MPP+-induced NF-κB suppression in differentiated ReNcell VM cells. These cells were transfected with NF-κB luciferase construct, infected with lenti-miR-7 or lenti-miR-SC vector, and treated with 1 mm MPP+ for 12 h. *p < 0.05, **p < 0.01. Relative percentage values are calculated against non-MPP+ controls.