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. 2014 Sep 17;34(38):12865–12876. doi: 10.1523/JNEUROSCI.1494-14.2014

Figure 6.

Figure 6.

FGFR signaling acts downstream of Hedgehog signaling in Hey1 and Hey2 regulation. A–I, FGFR signaling is required for SHH-mediated inhibition of HC differentiation. HC-specific Atoh1/nGFP reporter expression was monitored in E13.5 cochlear explants treated with vehicle control DMSO (A, E), FGFR inhibitor SU5402 (B, F), SHH + DMSO (C, G), and SU5402 + SHH (D, H) over a 24 h time period. Shown are low-power fluorescent images of HC-specific Atoh1/nGFP expression (GFP, white) at 12 h (A–D) and 24 h (E–H). Long arrow indicates basal-to-apical gradient; short arrow indicates medial-to-lateral gradient. I, Quantification of basal-to-apical extent of HC-specific Atoh1/nGFP expression in DMSO (dark gray bar), SHH + DMSO (purple bar), SU5402 (light gray bar), and SHH + SU5402 (white bar). Error bars indicate mean ± SEM (n = 5). *p ≤ 0.05. n.s., Not significant. J, FGFR signaling is required for SHH-mediated activation of Hey1 and Hey2 transcription. Shown is qPCR-based analysis of Hey1 and Hey2 mRNA levels in control (dark gray bar), SHH (purple bar), SU5402 (light gray bar), and SHH + SU5402 (white bar) treated cochlear epithelia after a 24 h treatment. Scale bar, 200 μm. Errors bars indicate mean ± SEM (n = 3, two independent experiments). *p ≤ 0.05. n.s., Not significant.