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. 2014 Sep 17;8:177. doi: 10.3389/fnsys.2014.00177

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Copyright © 2014 Bedwell, Billett, Crofts and Tinsley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Coronal section showing retrogradely labelled cells (blue) in cingulate cortex produced by 100 nl injection of Fluoro-gold into VLO (injection C). (B) Coronal section showing retrogradely labelled cells (blue) in cingulate cortex produced by 100 nl injection of Fluoro-gold into VO (injection B). (C) Coronal section showing anterograde labeling (brown) produced by injection of BDA (100 nl Fluorescein) into DLO (injection D). Arrow shows area of intense anterograde labeling of axon terminals. Other brown staining indicates less intense anterograde labeling, as well as some artifactual staining. Note the ordered location of labelled neurons within the dorsal-ventral axis. (D) Coronal section showing retrogradely labelled cells (blue) in sensory cortex produced by injections of Fluoro-Gold (100 nl) into VLO (injection C). (E) Coronal section showing retrogradely labelled cells (blue) in sensory cortex produced by injections of Fluoro-Gold (100 nl) into and VO (injection B). (F) Coronal section of sensory-motor cortex showing locations of anterograde labeling (brown) produced by injection of BDA (100 nl Texas red) into VLO (injection B). Arrow shows area of intense anterograde labeling of axon terminals. Other brown staining indicates less intense anterograde labeling, as well as some artifactual staining. Scale bars = 200 μm.