Table 2.
Characteristics of the DNA sequences obtained from excised RISA bands.
| Band name/accession number | Closest relative/Accession number | Coverage/identity | Length of analyzed DNA sequence | Estimated length of RIS amplicon (bp) |
|---|---|---|---|---|
| GP1-3 (JF729203) | uncultured Nitrosomonas (GQ330299) | 100/100 | 399 bp of RIS | 490 |
| GP1-5 (JF729204) | Thauera sp. MZ1T (CP001281) | 70/86 | 284 bp of RIS | 585 |
| GP1-6 (JF729205) | Uncultured Gemmatimonadaceae bacterium (HQ212581) | 100/82 | 150 bp of RIS + 129 bp of 23S rRNA gene | 150 |
| GP1-7 (JF729206) | Uncultured Actinobacterium clone BAL3SP-450-1 (HM124463) | 100/87 | 179 bp of RIS + 129 bp of 23S rRNA gene | 180 |
| GP1-12 (JF729207) | Thauera sp. MZ1T (CP001281) | 47/86 | 416 of RIS | 594 |
| GP2-1 (JF729208) | Thauera sp. MZ1T (CP001281) | 100/91 | 526 of RIS | 690 |
| GP2-3 (JF729209) | Bacillus cereus strain HY-1 (EU915686) | 99/100 | 115 bp of RIS + 131 bp of 23S rRNA gene | 200 |
| GP2-10 (JF729210) | Thauera sp. MZ1T (CP001281) | 100/91 | 526 of RIS | 670 |
| GP2-11 (JF729211) | Thauera sp. MZ1T (CP001281) | 100/87 | 535 of RIS | 526 |
| GP2-12 (JF729212) | Prochlorothrix scandica NIVA-8/90 (HQ316170) | 94/85 | 359 of RIS | 504 |
| GP3-1 (JF729213) | Paracoccus denitrificans PD1222 (CP000490) | 97/97 | 614 of RIS | 715 |