Table 3.

Nested-PCR for Mycobacterium tuberculosis complex and culture results of 159 bovine and bubaline tissue homogenates.

Status	Total number	Test	Number of positives (%)	p-value
CITT + and LCT	45	Nested-PCR +	32 (71.1)	0.318
		AFB + cultures*	37 (82.2)
CITT + and NVL	31	Nested-PCR +	17 (54.8)	0.124
		AFB + cultures*	10 (32.2)
CITT- and NVL	23	Nested-PCR +	0 (0.0)	0.999
		AFB + cultures*	1 (4.3)
No CITT and LCT	59	Nested-PCR +	33 (55.9)	0.015
		AFB + cultures*	19 (32.2)
No CITT and NVL	1	Nested-PCR +	0 (0.0)
		AFB + cultures*	1 (100.0)

CITT = Comparative intradermal tuberculin test. LCT = Lesions compatible with tuberculosis. NVL = no visible lesions. AFB= Acid-fast bacilli.

^*Confirmed by PCR with primers JB21 and JB22 (Rodriguez et al., 1995).