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. 2014 Sep 9;9:4293–4304. doi: 10.2147/IJN.S68012

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© 2014 Ho et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Effects of chitosan nanofibers on the induction of osteoblast differentiation-related OPN, OCN, and ALP gene expression.

Notes: Human osteoblast-like MG63 cells were seeded on chitosan nanofiber scaffolds for 1, 3, and 5 days. (A) Analyses of OPN, OCN, and ALP mRNAs were conducted using RT-PCR; β-actin mRNA was analyzed as an internal control. The DNA bands were quantified and statistically analyzed (B–D). Each value represents the mean ± standard error of the mean from four independent experiments. * indicates that values significantly differed from the respective control, P<0.05.

Abbreviations: ALP, alkaline phosphatase; mRNA, messenger RNA; OCN, osteocalcin; OPN, osteopontin; RT-PCR, reverse transcription polymerase chain reaction.