Table 1.

List of PCR primers used in this study.

Name	Sequence
rsbT fwd	cgcggatccatgaacgaccaatcctgtgtaag
rsbT rev	aaaactgcagctaccgaagccatttgatggcttg
hprk fwd	cgcggatccatggcaaaggttcgcacaaaag
hprk rev	aaaactgcagctattcttcttgttcaccgtcttc
rsbW fwd	cgcggatccatgaagaataatgctgattac
rsbW rev	aaaactgcagttagttagtttcgtagtttttga
ptkA fwd	cgcggatccatggcgcttagaaaaaacaga
ptkA rev	aaaactgcagttatttttgcatgaaattgtcc
prkD fwd	cgggatccatggcattaaaacttctaaaaaaactgc
prkD rev	aaaactgcagttatgtgaccgattgaatggcccg
yabT fwd	cgcagatctatgatgaacgacgctttgacgagt
yabT rev	ggactgcagtcacccacccgacttagccggtttct
prkC fwd	gaagatctatgctaatcggcaagcggatcagcgggcg
prkC rev	aaaactgcagttacaaaacccacggccacttttttctttttgccg
spoIIAB fwd	cgcggatccatgaaaaatgaaatgcaccttg
spoIIAB rev	aaaactgcagttaattacaaagcgctttgct
rsbV fwd	cgcggatccatgaatataaatgttgatgtg
rsbV rev	aaaactgcagtcattgcactccaccttct
spoAA fwd	cgcggatccatgagccttggaattgacatg
spoAA rev	aaaactgcagtcatgatgccaccccca
rsbS fwd	cgcggatccatgagacatccgaaaatcccga
rsbS rev	aaaactgcagctattcccccaattcccgctt
rsbT N49A fwd	ttagccagggctatttatttatatgccggcaaagggcagattg
rsbT N49A rev	taaataaatagccctggctaattctgaaatagccgttgtaattc
hprk K159M fwd	cggcgtcggaatgagcgaaacagcgctagagcttgtgaaaagag
hprk K159M rev	ctgtttcgctcattccgacgccgctttttcctgtgatcagcacg
rsbW N53A fwd	gcgtgcacagctgcggttcagcacgcttacaaagaagataaa
rsbW N53A rev	gctgaaccgcagctgtgcacgcctcactgactgcgattttc
ptkA K59D fwd	ggggaaggagattcaacaacggccgccaacctggctgtc
ptkA K59D rev	cgttgttgaatctccttcccccggacaagccgatgtaat
prkD K54D fwd	ttatgtcttagatcagcttcggccgacaaaagccaaaaag
prkD K54D rev	gccgaagctgatctaagacataaggtgtttgagctagg
YabT K55D fwd	tgttgccttagatgtgagtgatgacagcctgtctattac
YabT K55D rev	catcactcacatctaaggcaacatgtccatctgatgtt
prkC K40D fwd	agtcgcaattgatatcctgcggtttgactatgcaaatg
prkC K40D rev	caaaccgcaggatatcaattgcgacttcacggtctagaatg
spoIIAB N50A fwd	gctgtcacggctgcgattatccatggatatgaagagaactgtg
spoIIAB N50A rev	gataatcgcagccgtgacagcctctgacacgactgttttgat