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Western blot analyses of protein extracts from subcellular fractions of 3-week-old Arabidopsis using anti-peptide antibodies against ACBP4 and ACBP5.
Total whole plant protein (lane T), cytosol (lane C), large particles including mitochondria, chloroplasts and peroxisomes (lane L), nuclei (lane N), and membrane (lane M) subcellular fractions. Bottom, gel identically loaded was stained with Coomassie blue to show the protein amount in each lane.
