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. Author manuscript; available in PMC: 2014 Sep 18.
Published in final edited form as: Plant Mol Biol. 2008 Sep 5;68(6):571–583. doi: 10.1007/s11103-008-9392-7

Fig. 3.

Fig. 3

Constructs containing ACBP4- and ACBP5-tagged fusions.

(a) Generation of pACBP4∷DsRed, pACBP5∷DsRed and pGFP∷ACBP5 fusion constructs in which DsRed2 and GFP are translationally fused to amino acids 1 to 668 of ACBP4 and 1 to 648 of ACBP5, respectively.

(b) Generation of pGFP∷ACBP4, pACBP5∷GFP and pACBP5∷Red fusions. GFP from vector pBI-eGFP (Shi et al., 2005) translationally fused to the N-terminus of ACBP4 and the C-terminus of ACBP5 to generate pGFP∷ACBP4 and pACBP5∷GFP, respectively. The ACBP5∷DsRed fragment in (a) was cloned into binary vector pSa13, to generate pSa13-ACBP5∷DsRed (for differentiation, this fusion was designated as ACBP5∷Red).