Fig. 5.

Identification of transgenic Arabidopsis expressing GFP∷ACBP4, ACBP5∷GFP and ACBP5∷Red fusion proteins by northern blot and western blot analyses.

(a) Northern blot analysis of wild type and 3 independent transgenic plants transformed with GFP∷ACBP4. The 2.7-kbp GFP∷ACBP4 and the 2.0-kbp endogenous ACBP4 mRNAs are marked. Bottom, ethidium bromide-stained rRNA on the gel before blotting indicates the relative amounts of total RNA loaded per lane.

(b) Western blot analysis of wild type and 3 independent transgenic plants transformed with GFP∷ACBP4 show a 100.4-kDa GFP∷ACBP4 cross-reacting band. Bottom, protein gel identically loaded, stained with Coomassie blue.

(c) Northern blot analysis of wild type and 5 independent transgenic plants transformed with ACBP5∷Red. The 2.6-kbp ACBP5∷Red and the 1.9-kbp endogenous ACBP5 mRNAs are marked. Bottom, ethidium bromide-stained rRNA on the gel before blotting indicate the relative amounts of total RNA loaded per lane.

(d) Western blot analysis of wild type and transgenic lines #1, #3 and #5 transformed with ACBP5∷Red show a 99.5-kDa GFP∷ACBP4 cross-reacting band. Bottom, a protein gel identically loaded, stained with Coomassie blue.

(e) Northern blot analysis of wild type and 3 independent transgenic plants transformed with ACBP5∷GFP. The 2.6-kbp ACBP5∷GFP and the 1.9-kbp endogenous ACBP5 mRNAs are marked. Bottom, ethidium bromide-stained rRNA on the gel before blotting indicate the relative amounts of total RNA loaded per lane.

(f) Western blot analysis of wild type and transgenic lines #2 transformed with ACBP5∷GFP show a 98.5-kDa GFP∷ACBP4 cross-reacting band. Bottom, a protein gel identically loaded, stained with Coomassie blue.