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. 2014 Sep 2;2014:627181. doi: 10.1155/2014/627181

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Copyright © 2014 Xiaojia Ni et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tetramethylpyrazine (TMP) does not rescue endothelium-dependent relaxation (EDR) through actions on endothelial nitric oxide synthase (eNOS) expression. Rat aortic rings were subjected to various experimental conditions (phenylephrine (Phe); Phe plus acetylcholine (ACh); hydrogen peroxide (H₂O₂, 200 μM); TMP (10 nM) plus H₂O₂; losartan (1 μM) plus H₂O₂; or removal of the endothelium). The bar graph shows the ratio of the eNOS integrated density value (IDV) to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) IDV for each experimental condition. Data represent the mean ± the standard error of the mean (SEM) of 5–8 independent experiments.