Table 4.
DHE-mediated fluorescence microscopy assay in primary rat aortic endothelial cells.
| Treatment | Fluorescence intensity (% of control) | N |
|---|---|---|
| Control | 100 ± 0 | 7 |
| H2O2 (200 μM) | 158 ± 1.16∗ | 9 |
| TMP (10 nM) + H2O2 | 86 ± 2.10# | 5 |
| Apocynin (10 μM) + H2O2 | 88.30 ± 2.25# | 4 |
| DPI (10 nM) + H2O2 | 87.28 ± 5.40# | 3 |
| Tiron (100 μM) + DETCA (1 mM) + H2O2 | 67.95 ± 2.81# | 4 |
Data represent the means ± the SEM of 3–9 independent experiments (*P < 0.05 versus control; # P < 0.05 versus H2O2).
DHE: dihydroethidium; DETCA: diethyldithiocarbamic acid; DPI: diphenyleneiodonium; H2O2: hydrogen peroxide; SEM: standard error of the mean; TMP: tetramethylpyrazine.