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. 2014 Sep 16;9(9):e107743. doi: 10.1371/journal.pone.0107743

Figure 1. CytoBead ANCA assay principle.

Figure 1

Microscopic glass slides with ethanol-fixed human neutrophils (ethN; middle compartment of the well) and proteinase 3 (PR3) as well as myeloperoxidase (MPO) coated microbeads (right compartment of the well) are used for detection of anti-neutrophil cytoplasmic antibodies (ANCAs) by ethN-based indirect immunofluorescence and simultaneous analysis of PR3- and MPO-ANCA by microbead immunoassay. PR3-ANCA positive sera show cytoplasmic fluorescence patterns on ethN and a green fluorescence halo on the surface of PR3-coated microbeads (9 µm). In contrast, MPO-ANCA positive sera show perinuclear fluorescence patterns on ethN and a green fluorescence halo on the surface of MPO-coated microbeads (15 µm).