Table 2. Summary of altered cell cycle distribution in response to treatment with CSUOH0901(NSC751382).
SKBR-3 cells were treated for different time period with the indicated concentrations of compound. Cells were processed for FACS using propidium iodide staining as described. Percent distribution of cells in each cell cycle phase was displayed.
| Time | Concentrations of CSUOH0901 |
Sub-G1 % | G1 % | S % | G2/M % |
|---|---|---|---|---|---|
| 3h | 0.10(μM) | 3.12 | 70.84 | 17.20 | 8.83 |
| 0.25(μM) | 3.23 | 69.63 | 16.34 | 10.77 | |
| 0.50(μM) | 3.43 | 67.14 | 17.76 | 11.54 | |
| 5.0(μM) | 2.81 | 69.09 | 16.49 | 11.57 | |
|
| |||||
| 6h | 0.10(μM) | 3.60 | 71.43 | 15.84 | 9.07 |
| 0.25(μM) | 3.49 | 72.21 | 14.95 | 9.32 | |
| 0.50(μ M) | 4.03 | 67.10 | 14.73 | 13.90 | |
| 5.0(μM) | 3.42 | 64.76 | 14.61 | 17.19 | |
|
| |||||
| 12h | 0.10(μM) | 5.27 | 73.14 | 8.17 | 12.05 |
| 0.25(μM) | 6.85 | 73.62 | 6.14 | 13.35 | |
| 0.50(μM) | 6.36 | 63.15 | 5.94 | 24.26 | |
| 5.0(μM) | 9.76 | 63.71 | 5.99 | 20.54 | |
|
| |||||
| 18h | 0.10(μM) | 6.04 | 75.43 | 8.71 | 9.71 |
| 0.25(μM) | 8.89 | 73.42 | 6.81 | 10.57 | |
| 0.50(μM) | 8.47 | 61.19 | 7.28 | 22.27 | |
| 5.0(μM) | 8.31 | 63.55 | 6.73 | 21.80 | |
|
| |||||
| 24h | 0.10(μM) | 11.78 | 67.90 | 8.91 | 11.23 |
| 0.25(μM) | 24.95 | 41.27 | 10.87 | 22.53 | |
| 0.50(μM) | 27.94 | 31.77 | 11.21 | 28.14 | |
| 5.0(μM) | 27.62 | 31.87 | 11.53 | 28.29 | |