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. Author manuscript; available in PMC: 2014 Sep 18.
Published in final edited form as: J Pathol. 2014 Feb;232(3):308–318. doi: 10.1002/path.4294

Figure 6.

Figure 6

EZH2, KPNB1, and miR-30d expression correlates in human normal Schwann cells and MPNST cell lines. (A, B) qRT–PCR analyses of miR-30d and KPNB1 expression in human normal Schwann cells (NSCs) and MPNST cell lines MPNST724 (724), S462 (462), STS26T (26T), ST88-14 (ST88), T265 and MPNST642 (642). miR-30d and KPNB1 expression are normalized to SNORD47 and actin, respectively. Data are shown as mean ± SD; n = 3. (C) Western blot analyses for EZH2, KPNB1 and actin in human normal Schwann cells and in the MPNST cell lines MPNST724 (724), S462 (462), STS26T (26T), ST88-14 (ST88), MPNST642 (642) and T265. (D) Schematic model of EZH2-regulated miR-30d expression that targets KPNB1 in MPNST cells. EZH2 over-expression inhibits miR-30d transcription by binding to its promoter. Lower miR-30d expression results in KPNB1 up-regulation, which may increase the nuclear translocation of ERK proteins thereby promoting cell growth and survival.