Figure 1. mRNA profiles of adenylyl cyclase (AC) isoforms in human kidney cells.
mRNA was extracted from untransfected HEK-293 and hRPT cells from normotensive subjects using the RNeasy RNA Extraction Kit (Qiagen). RT-PCR was performed, as described previously (9). The sets of primers (A and B) used for human AC isoform mRNA detection are shown in Table S1, supplementary file.