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. Author manuscript; available in PMC: 2014 Sep 18.
Published in final edited form as: Dev Biol. 2014 Jun 28;393(1):3–9. doi: 10.1016/j.ydbio.2014.06.017

Fig. 2.

Fig. 2

Pigmentation phenotypes of mice resulting from Tyr CRISPR/Cas9 targeting by RNA injections into zygotes. (A) One albino (white arrowhead) and one pigmentation mosaic (yellow arrowhead) in one litter from RNA injections into C57BL/6N zygotes. (B) Two pigmentation mosaics with small patches of albino fur were found in another litter from injected C57BL/6N zygotes. C and D, Pups generated from RNA injections of (B6CBAF1/J × FVB/NJ) zygotes into pronudei (C) or cytoplasm (D). (E) Male pigmentation mosaic #1 (yellow asterisk) from the C57BL/6N injections was bred with an albino Swiss female to generate pups. Numerous albino progeny were obtained, demonstrating germ line transmission of the targeted Tyr mutant allele. (F) One of fully pigmented littermates from the C57BL/6N injections (yellow asterisk), a male, was bred with an albino Swiss female (black asterisk). Albino progeny were obtained, demonstrating germ line transmission of the targeted Tyr mutant allele.