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Binding activities of keratinocyte nuclear extracts as a function of differentiation state Keratinocyte nuclear extracts (NE) were prepared from SCC9 and SIK cultures which were preconfluent (Day -2), confluent (Day 0) or post-confluent (Days 7 and 14). Equal amounts of protein were incubated with TG-A and TG-G probes, and binding activities were determined by EMSA.
