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. 2014 Sep 16;9(9):e107495. doi: 10.1371/journal.pone.0107495

Figure 2. Phylogenetic relationships, exon-intron structure and protein domain analysis of MtIAA family genes.

Figure 2

(A) An unrooted phylogenetic tree was constructed using ClustalW by N-J method. The sister pair genes are indicated by red boxes. (B) Exon-intron structure analysis of MtIAA genes. The untranslated regions (UTRs) are indicated by thick red lines; the exons are indicated by blue boxes; the introns are indicated by gray lines. (C) Alignment of Medicago truncatula Aux/IAA proteins obtained with the ClustalW program and manual correction. Multiple alignments of the domains I–IV of the M. truncatula Aux/IAA proteins also were showed by red lines. Colorized shading indicates identical and conversed amino acid residues, respectively. The LXLXLX motif was also marked by thin yellow box. Two NLSs were marked by black asterisks.